Publications: Peer-reviewed journal articles (by staff)

Phenotypic biomarkers in selectively-bred families of the Greenshellâ„¢ mussel (Perna canaliculus): Anaerobic enzyme and shell gape behaviour as biomarkers of prolonged emersion tolerance

1 October, 2017
CITATION

Powell JA, Ragg NLC, Dunphy BJ 2017. Phenotypic biomarkers in selectively-bred families of the Greenshell™ mussel (Perna canaliculus): Anaerobic enzyme and shell gape behaviour as biomarkers of prolonged emersion tolerance. Aquaculture 479: 601-608.

DOI link here

ABSTRACT

Breeding Greenshell mussel (Perna canaliculus) families resilient to live shipment stress offers an opportunity to increase export revenue for the New Zealand mussel industry. We tested two key phenotypic responses (enzyme activity and shell gape) of selectively bred mussel families grouped (n = 3 × families/grouping) into those that showed ‘good’ (LD50 = 16.9–17.5 days), ‘average’ (LD50 = 13.3–13.9 days) or ‘poor’ (LD50 = 9.2–9.6 days) survivorship during a 30 day emersion trial. Mapping daily fold change of anaerobic enzyme activity revealed two phases in enzyme activity levels regardless of family. Specifically, an initial down-regulation of the enzymes alanopine dehydrogenase, and strombine dehydrogenase was observed in days 1–4 of the trial. This was then followed by marked up-regulation of enzyme activity, although the day at which up-regulation occurred varied among families; with ‘good’ surviving families up-regulating anaerobic metabolism earlier than either ‘average’ or ‘poor’ surviving families (i.e. 1–4 vs 6–9 and 6–8 days respectively). Such a result suggests that switching to anaerobic metabolism earlier confers greater tolerance to emersion stress. Peak level of enzyme activity, however, appears less important to withstanding prolonged emersion. [...]