Research Projects
Cawthron successfully collaborates with organisations around the world in the development of molecular tools for the detection and identification of marine organisms.
Recent collaboration includes:
| 1. |
Fluorescent in situ hybridization assays (FISH) for neurotoxic micro-algae based on the binding of fluorescently-labelled oligonucleotide probes to the 18S ribosomal RNA of the target species. Collaboration with Monterey Bay Aquarium Research Institute, California, and Florida Marine Research Institute, USA. This assay is also available for seastar (Asterias amurensis) larvae. |
| 2. |
qPCR-based detection is now being trialled for a range of toxic dinoflagellates including the paralytic shellfish toxin (PSP) producers, the Alexandrium group and Gymnodinium catenatum. |
| 3. |
A rapid automated system based on the sandwich hybridization assay (SHA), has been developed for fish-killing micro-algae - the main targets currently are the neurotoxin producers Karenia and the raphidophytes. The assay is also being developed for detection of marine pest larvae. |
Method Development and Validation
| 1. |
Novel methods for research projects |
| 2. |
Method Validation (ISO 17025) for regulated studies |
Routine Assays Available
| 1. |
FISH assay for Pseudo-nitzschias, Asterias amurensis |
| 2. |
SHA assay for raphidophytes, Karenias, Gymnodinium catenatum |
| 3. |
qPCR assay for Pfiesteria |
| 4. |
PCR assay for Karenias, Alexandrium and Gymnodinium catenatum |
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