Jonathan develops specialised testing methods to measure bioactives in a range of products and can assist with other analytical chemistry and biochemistry problems.
Role at Cawthron
Jonathan’s research focus is on harmful and helpful bioactives produced by microalgae. He works on toxic cyanobacteria (blue-green algae) which grow in waterways around New Zealand. This work is important as cyanobacteria pose a significant health risk to people and animals. Many other algae also produce beneficial bioactives and Jonathan is working to exploit these. Through this research, Jonathan aims to better understand the environmental factors which control bioactive production in algae.
Jonathan has a strong background in analytical chemistry and uses state-of-the-art liquid chromatography–mass spectrometry (LC-MS) equipment to assess a range of compounds. He also brings method development and trouble-shooting experience to Cawthron’s Analytical Services team.
- PhD in Chemistry. University of Waikato, 2012
- MSc in Molecular Biology (First Class Honours). University of Waikato, 2006
- BSc in Biology and Psychology. University of Waikato, 2004
Technical skills, experience and interests
- Analytical method development and trouble-shooting.
- Purification, characterisation, and formulation of beneficial bioactives from algae.
- Structural characterisation and identification of microcystins, a prevalent group of cyanobacterial toxins.
- Investigation into the triggers of toxin production in cyanobacteria.
View Jonathan's ResearchGate profile
- Murray JS, Selwood AI, van Ginkel, R, Puddick J, Rhodes L, Rise F and Wilkins A 2019. 44-Methylgambierone, a new gambierone analogue isolated from Gambierdiscus australes. Tetrahedron Letters.
- Steiner K, Wood SA, Puddick J, Hawes I, Dietrich DR and Hamilton DP 2017. A comparison of bacterial community structure, activity and microcystins associated with formation and breakdown of a cyanobacterial scum. Aquatic Microbial Ecology. Volume 80, Issue 3. Pages 243-256.
- Hodges CM, Wood SA, Puddick J and Hamilton DP 2017. Sensor manufacturer, temperature, and cyanobacteria morphology affect in situ phycocyanin fluorescence measurements. Environmental Science and Pollution Research.
- Wood SA and Puddick J 2017. The abundance of toxic genotypes is a key contributor to anatoxin variability in Phormidium-dominated benthic mats. Marine Drugs. Volume 15, Issue 10. Page 307.
- Wood SA, Maier MY, Puddick J, Pochon X, Zaiko A, Dietrich DR and Hamilton DP 2017. Trophic state and geographic gradients influence planktonic cyanobacterial diversity and distribution in New Zealand lakes. FEMS Microbiology Ecology. Volume 93, Issue 2.
- Wood SA, Puddick J, Fleming R and Heussner AH 2016. Detection of anatoxin-producing Phormidium in a New Zealand farm pond and an associated dog death. New Zealand Journal of Botany, DOI: 10.1080/0028825X.2016.1231122.
- Wood SA, Borges H, Puddick J, Biessy L, Atalah J, Hawes I, Dietrich DR and Hamilton DP 2016. Contrasting cyanobacterial communities and microcystin concentrations in summers with extreme weather events: Insights into potential effects of climate change. Hydrobiologia. Volume 785. Pages 71-89.
- Borges H, Wood SA, Puddick J, Blaney E, Hawes I, Dietrich DR and Hamilton DP 2016. Intracellular, environmental and biotic interactions influence recruitment of benthic Microcystis (Cyanophyceae) in a shallow eutrophic lake. Journal of Plankton Research. Volume 38, Issue 5. Pages 1289–1301.
- Puddick J, Wood SA and Hawes I, Hamilton DP 2016. Fine-scale cryogenic sampling of planktonic microbial communities: Application to toxic cyanobacterial blooms. Limnology and Oceanography: Methods. Volume 14. Pages 600-609.
- Puddick J, Prinsep MR, Wood SA, Cary SC and Hamilton DP 2016. Modulation of microcystin congener abundance following nitrogen depletion of a Microcystis batch culture. Aquatic Ecology. Volume 50, Issue 2. Pages 235–246.
- Rogers SR, Puddick J, Wood SA, Dietrich DR, Hamilton DP and Prinsep MR 2015. The effect of cyanobacterial biomass enrichment by centrifugation and GF/C filtration on subsequent microcystin measurement. Toxins, 7: Pages 821-834 .
- Puddick J, Prinsep MR, Wood SA, Cary SC and Holland PT 2015. Further characterization of glycine-containing microcystins from the McMurdo Dry Valleys of Antarctica. Toxins, 7: Pages 493-515.
- Puddick J, Prinsep MR, Wood SA, Kaufononga SAF, Cary SC and Hamilton DP 2014. High levels of structural diversity observed in microcystins from Microcystis CAWBG11 and characterization of six new microcystin congeners. Marine Drugs, 12: Pages 5372-5395.
- Kleinteich J, Wood SA, Puddick J, Schleheck D, Küpper FC and Dietrich D 2013. Potent toxins in Arctic environments; presence of saxitoxins and an unusual microcystin variant in Arctic freshwater ecosystems. Chemico-Biological Interactions. 206: Pages 423-431.
- Puddick J, Prinsep MR, Wood SA, Miles CO, Rise F, Cary SC, Hamilton DP and Wilkins A 2013. Structural characterization of new microcystins containing tryptophan and oxidized tryptophan residues. Marine Drugs. 11, 3025-3045.
- Puddick J, Prinsep MR, Wood SA, Cary SC and Hamilton D 2012. Enhanced sample preparation for quantitation of microcystins by matrix-assisted laser desorption/ionisation-time of flight mass spectrometry. Phytochemical Analysis 23: Pages 285-291.
- Tripathi A, Puddick J, Prinsep MR, Rottmann M and Tan LT 2010. Lagunamides A and B: Cytotoxic and antimalarial cyclodepsipeptides from the marine cyanobacterium Lyngbya majuscula. Journal of Natural Products. Volume 73. Pages 1810-1814.
- Tripathi A, Puddick J, Prinsep MR, Lee PPF and Tan LT 2008. Hantupeptin A, a cytotoxic cyclic depsipeptide from a Singapore collection of Lyngbya majuscula. Journal of Natural Products. Volume 72. Pages 29-32.
- Wood SA, Mountfort D, Selwood AI, Holland PT, Puddick J and Cary SC 2008. Widespread distribution and identification of eight novel microcystins in Antarctic cyanobacterial mats. Applied and Environmental Microbiology. Volume 74. Pages 7243-7251.